abstract：:DNA samples of 2303 individuals from nine different population groups were screened for variant -175g-->t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The -175g-->t variant detected at carrier frequencies of 3-10% in different African population groups was absent in the Caucasian and Asi...

journal_title：Molecular and cellular probes

authors： Hoogendijk CF,Scholtz CL,Pimstone SM,Ehrenborg E,Kastelein JJ,Defesche JC,Thiart R,du Plessis L,de Villiers JN,Zaahl MG,Delport R,Rubinsztein DC,Raffel LJ,Grim CE,Mediene-Benchekor S,Amouyel P,Brousseau T,Steyn K,Lomb

更新日期：2003-08-01 00:00:00

abstract：:A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hyb...

journal_title：Molecular and cellular probes

authors： Poddar SK

更新日期：2003-04-01 00:00:00

abstract：:The cellular retinoic acid binding protein-II (CRABP-II) is an intracellular protein involved in the transmission of the vitamin A-derived signal which regulates genes responsible for lipid metabolism and adipocyte differentiation. Cellular Retinoic Acid Binding Protein-II gene (CRABP-II) (GDB 134819) is located on ch...

journal_title：Molecular and cellular probes

authors： Salazar J,Ferré R,Vallvé JC,Pocoví M,Cabezas MC,Masana L,Ribalta J

更新日期：2003-02-01 00:00:00

abstract：:The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

journal_title：Molecular and cellular probes

authors： Cancilla PA,Barrette P,Rosenblum F

更新日期：2002-12-01 00:00:00

abstract：:Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

journal_title：Molecular and cellular probes

authors： Bang DD,Wedderkopp A,Pedersen K,Madsen M

更新日期：2002-10-01 00:00:00

abstract：:The identification of Dichelobacter nodosus present in a flock is a prerequisite to specific (autogenous) vaccination. Current methods of identification of the serogroup present in a population requires that the organisms be isolated, identified visually in mixed culture on streak plates, subcultured to purify and sub...

journal_title：Molecular and cellular probes

authors： Dhungyel OP,Whittington RJ,Egerton JR

更新日期：2002-08-01 00:00:00

abstract：:Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

journal_title：Molecular and cellular probes

authors： Bhaduri S

更新日期：2002-06-01 00:00:00

abstract：:Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

journal_title：Molecular and cellular probes

authors： Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

更新日期：2002-04-01 00:00:00

abstract：:The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs i...

journal_title：Molecular and cellular probes

authors： Nagaraju J,Kathirvel M,Subbaiah EV,Muthulakshmi M,Kumar LD

更新日期：2002-02-01 00:00:00

abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

journal_title：Molecular and cellular probes

authors： French DJ,Archard CL,Brown T,McDowell DG

更新日期：2001-12-01 00:00:00

abstract：:The progressive myoclonus epilepsy of Lafora type (LD) is an autosomal recessive disorder caused by mutations in the EPM2A gene. We demonstrated recently that EPM2A encodes a dual-specificity phosphatase that is primarily associated with polyribosomes. In the present study, we screened for mutations in the EPM2A gene ...

journal_title：Molecular and cellular probes

authors： Ganesh S,Shoda K,Amano K,Uchiyama A,Kumada S,Moriyama N,Hirose S,Yamakawa K

更新日期：2001-10-01 00:00:00

abstract：:A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

journal_title：Molecular and cellular probes

authors： Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

更新日期：2001-08-01 00:00:00

abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

journal_title：Molecular and cellular probes

authors： Chaubert P,Burri N,Cousin P,Shaw P

更新日期：2001-06-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00

abstract：:The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

journal_title：Molecular and cellular probes

authors： Okajima K,Paznekas WA,Burstyn T,Jabs EW

更新日期：2001-02-01 00:00:00

abstract：:The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

journal_title：Molecular and cellular probes

authors： Drewe LJ,Brightwell G,Hall EA

更新日期：2000-10-01 00:00:00

abstract：:Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

journal_title：Molecular and cellular probes

authors： Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

更新日期：2000-08-01 00:00:00

abstract：:Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the sa...

journal_title：Molecular and cellular probes

authors： Winters DK,Slavik MF

更新日期：2000-04-01 00:00:00

abstract：:One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA. PCR with this primer pair amplified only the DNA of C. parvum, not the control DNA including Cryptosporidium muris. This primer pair had most advantag...

journal_title：Molecular and cellular probes

authors： Wu Z,Nagano I,Matsuo A,Uga S,Kimata I,Iseki M,Takahashi Y

更新日期：2000-02-01 00:00:00

abstract：:A comparative analysis of the two most dominant erythromycin-resistance determinant genes in Staphylococcus sppnamely, the ermA and ermC genes, was carried out. Sixty erythromycin-resistant strains of Staphylococcus spp. were tested, of which 24 were avian and 36 were clinical isolates. Our results indicated the preva...

journal_title：Molecular and cellular probes

authors： Khan SA,Nawaz MS,Khan AA,Cerniglia CE

更新日期：1999-10-01 00:00:00

abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

journal_title：Molecular and cellular probes

authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

更新日期：1999-08-01 00:00:00

abstract：:Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

journal_title：Molecular and cellular probes

authors： Owshalimpur D,Kelley MJ

更新日期：1999-06-01 00:00:00

abstract：:The ability to rapidly and reliably genotype mice is an important concern. Traditional methods employ labour intensive and time consuming techniques such as test crossing, gel electrophoresis or nucleic acid hybridization. Here we show that a new molecular biology workstation, the WAVE DNA Fragment Analysis System, ca...

journal_title：Molecular and cellular probes

authors： Kuklin A,Davis AP,Hecker KH,Gjerde DT,Taylor PD

更新日期：1999-06-01 00:00:00

abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

journal_title：Molecular and cellular probes

authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

更新日期：1999-04-01 00:00:00

abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

journal_title：Molecular and cellular probes

authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

更新日期：1999-02-01 00:00:00

abstract：:The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This...

journal_title：Molecular and cellular probes

authors： Lucotte G,Mercier G,Burckel A

更新日期：1998-12-01 00:00:00

abstract：:An example of the application and contruction of a polymerase chain reaction (PCR) internal control is presented. The internal control is synthesized in one PCR reaction. The primers used in this reaction possess 5' over-hanging ends which are identical to the primers used in the diagnostic reaction, whereas their 3' ...

journal_title：Molecular and cellular probes

authors： Sachadyn P,Kur J

更新日期：1998-10-01 00:00:00

abstract：:For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

journal_title：Molecular and cellular probes

authors： Ursi D,Ieven M,Van Bever HP,Goossens H

更新日期：1998-08-01 00:00:00

abstract：:Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region...

journal_title：Molecular and cellular probes

authors： Castignolles N,Petit F,Mendel I,Simon L,Cattolico L,Buffet-Janvresse C

更新日期：1998-06-01 00:00:00

abstract：:The hydroxymethylbilane synthase (HMBS) mRNAs from 44 control individuals and 30 patients suffering from acute intermittent porphyria (AIP), were screened for length differences by reverse transcriptase polymerase chain reaction (RT-PCR) and any abnormalities were characterized by direct sequencing. Examination of the...

journal_title：Molecular and cellular probes

authors： Ong PM,Lanyon WG,Moore MR,Connor JM

更新日期：1998-04-01 00:00:00

abstract：:Transcription of human papillomavirus (HPV) type 33 early region was analysed in the UT-DEC-1 keratinocyte cell line, which has been derived from a HPV-33-containing mild vaginal dysplasia. Fifteen cDNA clones from transcripts from the E6-E7 open reading frames were constructed and analysed. Most clones represented vi...

journal_title：Molecular and cellular probes

authors： Auvinen E,Hietanen S,Gissmann L,Alonso A

更新日期：1998-02-01 00:00:00

abstract：:A simple assay format was developed for the direct detection of C. trachomatis rRNA utilizing ligation of recombinant MDV-1 probe RNA fragments hybridized to 23S rRNA after capture and release from a solid support. Assay background (equivalent to 10(4) targets) was suppressed by blocking sequences in the 5' MDV report...

journal_title：Molecular and cellular probes

authors： Stefano JE,Genovese L,An Q,Lu L,McCarty J,Du Y,Stefano K,Burg JL,King W,Lane DJ

更新日期：1997-12-01 00:00:00

abstract：:A polymerase chain reaction (PCR) protocol was developed that could specifically amplify a 520-bp region of the erythromycin resistant methylase (ermC) gene sequence. The identity of the PCR-amplified 520-bp DNA was confirmed by HinCII endonuclease restriction digestion, which produced the predicted 440-bp and 80-bp D...

journal_title：Molecular and cellular probes

authors： Nawaz MS,Khan AA,Cerniglia CE

更新日期：1997-10-01 00:00:00

abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

journal_title：Molecular and cellular probes

authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

更新日期：1997-08-01 00:00:00

abstract：:To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

journal_title：Molecular and cellular probes

authors： Lucotte G,Burckel A

更新日期：1997-08-01 00:00:00

abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

journal_title：Molecular and cellular probes

authors： Wang H,Fadl AA,Khan MI

更新日期：1997-06-01 00:00:00

abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

journal_title：Molecular and cellular probes

authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

更新日期：1997-04-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

journal_title：Molecular and cellular probes

authors： Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

更新日期：1997-02-01 00:00:00

abstract：:Wild-type p53 (wtp53) is a tumour suppressor gene involved in cell cycle regulation. The mdm2 protein can complex with the p53 protein and influence its function as a regulator of cell growth. To detect and quantify wtp53 and mdm2 mRNA expression, we established the competitive reverse transcription/polymerase chain r...

journal_title：Molecular and cellular probes

authors： Totzke G,Sachinidis A,Vetter H,Ko Y

更新日期：1996-12-01 00:00:00

abstract：:A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

journal_title：Molecular and cellular probes

authors： Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

更新日期：1996-10-01 00:00:00